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KMID : 0895820140240040015
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Journal of Oriental Rehabilitation Medicine
2014 Volume.24 No. 4 p.15 ~ p.28
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Study on Antioxidant and Anti-inflammatory Effects of Components of Mahwangbujaseshin-tang
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Choi Chul-Woo
Oh Min-Seok
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Abstract
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Objectives : This study was carried out to find out the Antioxidant and Anti-inflammatory Effects of Components of Mahwangbujaseshin-tang in LPS-Stimulated RAW264.7 Macrophages.
Methods : There are 5 experimental groups. ; normal, control, EH (Ephedrae Herba), ALRP (Aconiti Lateralis Radix Preparata) and AR (Asiasari Radix). The extract of EH, ALRP and AR (100 μg/ml) was added to each group. We examined cytotoxicity, total phenolic contents, DPPH and ABTS free radical scavenging activity, Intracellular ROS (reactive oxy-gen species) production, NO (Total Nitric oxide), iNOS (inducible nitric oxide synthase), PGE2 (prostaglandin E2), COX-2 (cyclooxygenase-2), IL-1β (interleukin-1β), IL-6 (inter-leukin-6), TNF-α (tumor necrosis factor-α), MMP-9 (matrix metalloproteinase-9), TIMP-1 (tissue inhibitor of metalloproteinase-1) and HO-1 (heme oxygenase-1) expression level.
Results : 1. Total phenolic contents of EH were in the highest level. 2. DPPH and ABTS free radical scavenging activity of EH was in the highest level. 3. ROS production was sig-nificantly decreased in AR. 4. NO production was significantly decreased in EH, ALRP, AR and iNOS expression was decreased in EH, AR. 5. PGE2 and COX-2 expression was de-creased in EH, AR. 6. IL-1β production was significantly decreased in EH, AR and IL-6 production was significantly decreased in AR. TNF-α production was significantly de-creased in ALRP, AR. 7. MMP-9 and TIMP-1 production were significantly decreased in EH. 8. HO-1 expression was significantly increased in EH. 9. With simultaneous usage of SnPP which is expression inhibitor of HO-1, NO, IL-1β, IL-6 and TNF-α production were partially increased in EH, ALRP, AR.
Conclusions : According to this study, Components of Mahwangbujaseshin-tang have anti-oxidants and anti-inflammation effects in LPS-Stimulated RAW264.7 Macrophages.
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KEYWORD
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Mahwangbujaseshin-tang, Anti-inflammation, Anti-oxidation
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